Reproduction Abstracts

Premature ovarian failure (POF) affects ~1% of women over 40 and is idiopathic in 74–90% of cases. A transgenic mouse model of POF has been generated (known as double mutant; DM) resulting from oocyte-specific deletion of two glycosyltransferases. Glycoproteins from DM oocytes lack complex O- and N-glycans. DM females are fertile at 6-weeks, infertile by 9-weeks and exhibit POF by 12-weeks of age with follicle depletion, elevated gonadotropins and decrea...

Premature ovarian failure (POF) affects around 1% of women and is idiopathic in 74–90% of cases. Our mouse model of POF, the double mutant (DM), are fertile at 6-weeks and undergo POF by 3-months consistent with a decline in developing follicles. However, DM ovaries have increased numbers of primary follicles at the 3a stage, indicating a block in follicle development.We investigated whether we could rescue the ability of DM oocytes at 3- and 9-week...

Introduction: Premature ovarian failure (POF) is a condition that affects ~1% of women and is idiopathic in 74–90% of cases. Our mouse model of POF, the Double Mutant (DM), results from oocyte-specific ablation of core 1-derivedO-glycans and complex and hybrid N-glycans. DM females are subfertile at 6-weeks of age and infertile at 9-weeks of age. By 3 months, DM females exhibit POF with ovaries containing fewer developing follicles but more primary 3a follicles. We invest...